Cognitive / Nootropic

PE-22-28 Peptide Protocol & Dosing

Updated 2mo ago 5 min read 195+ helpful Calculator

Validity Score

16 Speculative

Evidence 21

Community 35

Completeness 71

Actionability 80

Coverage 80

Penalty -26

This score combines formal evidence, page completeness, calculator readiness, and community convergence.

What it is

PE-22-28 is a preclinical spadin-derived peptide that blocks TREK-1 potassium channels and shows antidepressant-like effects in animal models. Human trials are not available.

Evidence boundary

Preclinical / speculative. Do not present a human dosing schedule as established fact.

Trial evidence

No verified human trial found.

Preparation and measurement

Reconstitution: 10 mg vial + 2 mL BAC = 5 mg/mL.

Concentration matches.

Reference schedule

Reference schedule
Phase	Dose	Draw / measure	Frequency	Weekly total
Weeks 1–2 (titration)	50 mcg	1 U (0.01 mL)	Daily during cycle	350 mcg/week
Weeks 3–8 (target)	100 mcg	2 U (0.02 mL)	Daily during cycle	700 mcg/week
Weeks 9–12 (optional)	150 mcg	3 U (0.03 mL)	Daily during cycle	1.05 mg/week
Weeks 13–16 (optional)	200 mcg	4 U (0.04 mL)	Daily during cycle	1.4 mg/week

Weeks 1–2 (titration)

Dose: 50 mcg
Draw / measure: 1 U (0.01 mL)
Frequency: Daily during cycle
Weekly total: 350 mcg/week

Weeks 3–8 (target)

Dose: 100 mcg
Draw / measure: 2 U (0.02 mL)
Frequency: Daily during cycle
Weekly total: 700 mcg/week

Weeks 9–12 (optional)

Dose: 150 mcg
Draw / measure: 3 U (0.03 mL)
Frequency: Daily during cycle
Weekly total: 1.05 mg/week

Weeks 13–16 (optional)

Dose: 200 mcg
Draw / measure: 4 U (0.04 mL)
Frequency: Daily during cycle
Weekly total: 1.4 mg/week

Weekly totals apply only during the active phase.

Titration rules

Daily titration (subcutaneous):
Weeks 1–2 (titration): 50 mcg/day.
Weeks 3–8 (target): 100 mcg/day. Common research dose.
Weeks 9–12 (optional): 150 mcg/day.
Weeks 13–16 (optional): 200 mcg/day. Upper end of researched range.
TREK-1 mechanism in the source literature
Limited human data

Practical rules

Pair with established mental-health care if relevant.
Do not present the dose ladder as validated human guidance.

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Dosing references

Peptide dosage chart Peptide dose calculator Syringe mixability

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Dosing Calculator

10 mg + 2 mL BAC · week 1 50 mcg

LIVE CALCULATIONvolume / concentration / draw

RP–CALC–01

01 Vial size

02 BAC water

DRAW AMOUNT1 units0.01 mL · U-100

Draw 1 units, equivalent to 0.01 milliliters.

Concentration5 mg/mL

Selected dose0.05 mg (50 mcg) = 1 units

04 Custom dose

05 Standard amounts

1–10 mg · 0.5 mg steps

06 Suggested doses Select a row

1 unit = 0.01 mL on a U-100 syringe. Values retain decimal precision; visual marks are not silently snapped.

07 cycle summary

Vials required for Cycle

Estimate how much material you need for a full cycle, including cycle length and the number of cycles you plan to run.

Cycle length (weeks) Injections per week Number of cycles

Cycle total —

Total required vials —

Total reconstitution —

Total BAC water —

08 projected levels

How the draws stack up

A first-order model of depot absorption and elimination, run on the draws and cadence above. Pick the half-life from whatever your own source documents — this page does not assume one per compound. A planning sketch — not a prediction for any compound or any individual.

Half-life

Hours

Modelled level across the cycle. Each spike is one draw.

Accumulation—steady state vs one draw

Peak-to-trough—inside one interval

Steady state—about 4 half-lives

Draws modelled—at this cadence

Vial yieldat the selected amount

Draws per vial—

Lasts—

Volume used—

Limiting factor—

Water matrixsame vial, same amount

WaterStrengthDraw

0.5 mL——

1 mL——

2 mL——

3 mL——

5 mL——