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Longevity / Anti-Aging

SS-31 (Elamipretide) Peptide Protocol & Dosing

Updated 1mo ago 6 min read 248+ helpful Calculator
Validity Score

Validity Score

68 Usable
Evidence 86
Community 70
Completeness 57
Actionability 60
Coverage 100
Penalty -8

This score combines formal evidence, page completeness, calculator readiness, and community convergence.

What it is

SS-31, also called elamipretide, is a mitochondria-targeted peptide studied for cardiolipin binding and mitochondrial function.

Evidence boundary

Investigational with human trial data.

Trial evidence

  • PMID 42440741 — Therapeutic targeting of mitochondrial dysfunction in heart failure: a systematic review & meta-analysis of clinical outcomes.
  • PMID 35165982 — Effect of mitochondrial-targeted antioxidants on glycaemic control, cardiovascular health, and oxidative stress in humans: A systematic review and meta-analysis of randomized controlled trials.
  • PMID 42123471 — Therapeutic Peptides in Aesthetic, Metabolic and Endocrine Conditions: Effects, Safety, Clinical Applications, and Future Perspectives.

Preparation and measurement

Reconstitution: 50 mg vial + 1 mL BAC = 50 mg/mL.

Concentration matches.

Reference schedule

Reference schedule
PhaseDoseDraw / measureFrequencyWeekly total
Weeks 1–2 (titration)5 mg10 U (0.1 mL)Once daily35 mg/week
Weeks 3–8 (target)10 mg20 U (0.2 mL)Once daily70 mg/week

Weeks 1–2 (titration)

Dose
5 mg
Draw / measure
10 U (0.1 mL)
Frequency
Once daily
Weekly total
35 mg/week

Weeks 3–8 (target)

Dose
10 mg
Draw / measure
20 U (0.2 mL)
Frequency
Once daily
Weekly total
70 mg/week

Weekly totals apply only during the active phase.

Titration rules

  • Daily titration (subcutaneous):
  • Weeks 1–2 (titration): 5 mg/day.
  • Weeks 3–8 (target): 10 mg/day. Aligned with trial doses used in cardiac and ophthalmic studies.
  • Cycle structure: 12–24 weeks continuous. Effects accumulate gradually; expect 8–12 weeks before subjective changes.

Cycle length and breaks

Effects accumulate gradually; expect 8–12 weeks before subjective changes.

What to monitor

  • Mitochondrial and cardiac response
  • Energy and fatigue signals over time
  • Injection-site reactions

Practical rules

  • Rotate sites.
  • Do not frame this as a finished standard therapy.
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Dosing Calculator

10 mg + 2 mL BAC · suggested 1 mg
LIVE CALCULATIONvolume / concentration / draw
RP–CALC–01
DRAW AMOUNT5 units0.05 mL · U-100
Draw 5 units, equivalent to 0.05 milliliters.
Concentration5 mg/mL
Selected dose0.25 mg (250 mcg) = 5 units
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05 Standard doses
06 Suggested doses Select a row
1 unit = 0.01 mL on a U-100 syringe. Values retain decimal precision; visual marks are not silently snapped.
07 cycle summary

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