All protocols
Longevity / Anti-Aging

Epitalon Peptide Protocol & Dosing

Updated 2mo ago 6 min read 356+ helpful Calculator
Validity Score

Validity Score

13 Speculative
Evidence 21
Community 35
Completeness 57
Actionability 60
Coverage 100
Penalty -26

This score combines formal evidence, page completeness, calculator readiness, and community convergence.

What it is

Epitalon is a synthetic tetrapeptide from pineal-extract work.

Evidence boundary

Regional / investigational.

Trial evidence

  • PMID 40908429 — Epitalon increases telomere length in human cell lines through telomerase upregulation or ALT activity.
  • PMID 40493162 — The Antioxidant Tetrapeptide Epitalon Enhances Delayed Wound Healing in an In Vitro Model of Diabetic Retinopathy.
  • PMID 40141333 — Overview of Epitalon-Highly Bioactive Pineal Tetrapeptide with Promising Properties.
  • PMID 42021992 — Therapeutic peptides in gerontology: mechanisms and applications for healthy aging.

Preparation and measurement

Reconstitution: 10 mg vial + 1 mL BAC = 10 mg/mL.

Concentration matches.

Reference schedule

Reference schedule
PhaseDoseDraw / measureFrequencyWeekly total
Microgram protocol, active days 1–10100–500 mcg1–5 U (0.01–0.05 mL)Once daily0.7–3.5 mg/week while active
Microgram protocol, days 11–3000 U (no draw)Off0
Classic protocol5 mg50 U (0.5 mL)Once daily for 10–20 days35 mg/week while active
Classic high-dose variant10 mg100 U (1 mL)Once daily for 10 days70 mg/week while active

Microgram protocol, active days 1–10

Dose
100–500 mcg
Draw / measure
1–5 U (0.01–0.05 mL)
Frequency
Once daily
Weekly total
0.7–3.5 mg/week while active

Microgram protocol, days 11–30

Dose
0
Draw / measure
0 U (no draw)
Frequency
Off
Weekly total
0

Classic protocol

Dose
5 mg
Draw / measure
50 U (0.5 mL)
Frequency
Once daily for 10–20 days
Weekly total
35 mg/week while active

Classic high-dose variant

Dose
10 mg
Draw / measure
100 U (1 mL)
Frequency
Once daily for 10 days
Weekly total
70 mg/week while active

Weekly totals apply only during the active phase.

Titration rules

  • These are separate protocol families, not steps in one escalation.
  • The microgram protocol uses 100–500 mcg for 10 days, then 20 days off.
  • The classic protocol uses 5–10 mg/day for 10–20 days and is repeated only once or twice per year.

Cycle length and breaks

Do not mix milligram and microgram rows within the same cycle.

What to monitor

  • Telomere / telomerase claims in the literature
  • Circadian and sleep-related claims
  • Tolerability
  • Injection-site reactions

Practical rules

  • Do not present the dose question as validated.
  • Rotate sites if it is being compared in notes.
Was this protocol helpful?
Share via

Dosing references

Peptide dosage chart Peptide dose calculator Syringe mixability

Related protocols

SS-31 (Elamipretide) Peptide Protocol & DosingMOTS-c Peptide Protocol & DosingNAD+ Protocol & DosingPinealon Peptide Protocol & DosingHumanin Peptide Protocol & Dosing
Supply & lot verification Availability and batch-linked COA records for Epitalon Peptide Protocol & Dosing are tracked with the current supplier listings. View availability & COA →

Dosing Calculator

10 mg + 2 mL BAC · week 1 100 mcg
LIVE CALCULATIONvolume / concentration / draw
RP–CALC–01
DRAW AMOUNT2 units0.02 mL · U-100
Draw 2 units, equivalent to 0.02 milliliters.
Concentration5 mg/mL
Selected dose0.1 mg (100 mcg) = 2 units
Enter a desired amount in milligrams or micrograms.
05 Standard amounts
1–10 mg · 0.5 mg steps
06 Suggested doses Select a row
1 unit = 0.01 mL on a U-100 syringe. Values retain decimal precision; visual marks are not silently snapped.
07 cycle summary

Vials required for Cycle

Estimate how much material you need for a full cycle, including cycle length and the number of cycles you plan to run.

Cycle total —
Total required vials —
Total reconstitution —
Total BAC water —
08 projected levels

How the draws stack up

A first-order model of depot absorption and elimination, run on the draws and cadence above. Pick the half-life from whatever your own source documents — this page does not assume one per compound. A planning sketch — not a prediction for any compound or any individual.

Half-life

Modelled level across the cycle. Each spike is one draw.

Accumulation—steady state vs one draw
Peak-to-trough—inside one interval
Steady state—about 4 half-lives
Draws modelled—at this cadence
Vial yieldat the selected amount
Draws per vial—
Lasts—
Volume used—
Limiting factor—
Water matrixsame vial, same amount
WaterStrengthDraw
0.5 mL——
1 mL——
2 mL——
3 mL——
5 mL——

Research Protocols

Keep up to date with latest protocols, updates and offers

An occasional email when a new protocol lands or an existing one is revised. No more than once a month.